Genome-wide SNP-discovery and analysis of genetic diversity in oil palm using double digest restriction site associated DNA sequencing
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چکیده
منابع مشابه
Genome-Wide SNP Discovery and Analysis of Genetic Diversity in Farmed Sika Deer (Cervus nippon) in Northeast China Using Double-Digest Restriction Site-Associated DNA Sequencing
Sika deer are an economically valuable species owing to their use in traditional Chinese medicine, particularly their velvet antlers. Sika deer in northeast China are mostly farmed in enclosure. Therefore, genetic management of farmed sika deer would benefit from detailed knowledge of their genetic diversity. In this study, we generated over 1.45 billion high-quality paired-end reads (288 Gbp) ...
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Mapping of quantitative trait loci (QTL) is essential for the discovery of genetic structures that related to complex quantitative traits. In this study, we identified 264,072 raw SNPs (single-nucleotide polymorphisms) by double digest restriction site associated DNA sequencing (ddRADseq), and utilized 3029 of these SNPs to construct a genetic linkage map in orange-spotted grouper (Epinephelus ...
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DNA methylation is a type of epigenetic changes that directly affects DNA. In mammals, DNA methylation is essential for fetal development and stem cell differentiation and this phenomenon essentially occurs within the CpG islands. In this study, two methods were used to study the DNA methylation profile of cow genome. In the first method, the DNA methylation profile of the differentially expres...
متن کاملAFSM sequencing approach: a simple and rapid method for genome-wide SNP and methylation site discovery and genetic mapping
We describe methods for the assessment of amplified-fragment single nucleotide polymorphism and methylation (AFSM) sites using a quick and simple molecular marker-assisted breeding strategy based on the use of two restriction enzyme pairs (EcoRI-MspI and EcoRI-HpaII) and a next-generation sequencing platform. Two sets of 85 adapter pairs were developed to concurrently identify SNPs, indels and ...
متن کاملGenome-wide genotyping and SNP discovery by ultra-deep Restriction-Associated DNA (RAD) tag sequencing of pooled samples of E. grandis and E. globulus
Background The availability of next generation sequencing (NGS) technologies has opened the door to new strategies of SNP discovery and genotyping. Rapid genome-wide SNP detection via deep resequencing of reduced representation libraries of restriction digested pools of genomic DNA combined with a reference genome has been successfully used for SNP discovery in microorganisms [1], plants [2]and...
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ژورنال
عنوان ژورنال: IOP Conference Series: Earth and Environmental Science
سال: 2019
ISSN: 1755-1315
DOI: 10.1088/1755-1315/293/1/012041